路氏锥虫复合表面被膜的糖蛋白

The glycoproteins of the complex surface coat of Trypanosoma lewisi.

作者信息

Battaglia P A, Zani B, Del Bue M, Ponzi M, Birago C

出版信息

Cell Biol Int Rep. 1983 Sep;7(9):755-62. doi: 10.1016/0309-1651(83)90203-5.

Abstract

Blood stream forms of Trypanosoma lewisi from rats previously infected were labelled in vitro by galactose-oxidase oxidation followed by NaB3H4 reduction and subjected to 7.5% SDS-polyacrylamide gel analysis, by this procedure 8 bands were detected on SDS polyacrylamide gel electrophoresis. We conclude that surface composition of Trypanosoma lewisi resulted more complex than that of Trypanosoma so far studied, suggesting a possible relation with induced immunity and reduced ability of the parasite to survive in its host.

摘要

对先前感染过的大鼠体内的路氏锥虫血流型进行体外标记，先用半乳糖氧化酶氧化，再用硼氢化钠还原，然后进行7.5%十二烷基硫酸钠-聚丙烯酰胺凝胶分析，通过该程序在十二烷基硫酸钠聚丙烯酰胺凝胶电泳上检测到8条带。我们得出结论，路氏锥虫的表面组成比迄今为止研究过的锥虫更为复杂，这表明它与诱导免疫以及寄生虫在宿主中生存能力的降低可能存在关联。