Müller F, Lindenschmidt E G
Hautarzt. 1983 Sep;34(9):448-52.
Approximately 10% of the investigated 49 patients with primary syphilis are non-reactive in the TPHA test if the standard technique is used. The reason for this observation can be elucidated by the ELISA technique. Only patients are affected in a second phase of immunological defence which is characterized by identical serum titres of treponemal IgM and IgG antibodies. The rate of TPHA false non-reactive results in primary syphilis can be diminished by using the test with a starting serum dilution of 1:5 instead of 1:20.
如果采用标准技术，在49例接受调查的一期梅毒患者中，约10%的患者在梅毒螺旋体血凝试验（TPHA）中呈阴性反应。这一观察结果的原因可用酶联免疫吸附测定（ELISA）技术来阐明。只有患者在免疫防御的第二阶段受到影响，其特征是梅毒螺旋体IgM和IgG抗体的血清滴度相同。通过使用起始血清稀释度为1:5而非1:20的试验，一期梅毒中TPHA假阴性结果的发生率可以降低。
