Excretion of neutral alpha-glucosidase, determined with a continuous assay, and of acid alpha-glucosidase in the urine of human reference subjects.

The catalytic activities of neutral and acid alpha-glucosidase were selectively determined in human urine. Urinary excretion of neutral and acid alpha-glucosidase in reference subjects was found to be in the range 1.61 to 20.36 microkat/mol creatinine and 7.47 to 33.60 microkat/mol creatinine, respectively. Urinary excretion of both enzymes was not related to sex, age or diuresis. A continuous assay was introduced to improve the determination of neutral alpha-glucosidase.