Sang D N, Anderson D J
Retina. 1983;3(3):206-11.
The full expression of Class 1 antigens of the major histocompatibility complex (HLA A,B,C,) on ocular cell surfaces appears to be developmentally regulated; HLA is undetectable in retina from 16-week-old fetal eyes but is present in adult retina. Cultured retinoblastoma (RB) may serve as a useful model of retinal cell development. SF81 is an undifferentiated subculture of in vitro retinoblastoma with little evidence of spontaneous differentiation and lack of expression of HLA, attesting to the "undifferentiated" character of this cell line. RB SF81 cells were treated with retinoic acid, a known stimulator of differentiation (optimal concentration 5 X 10(-8) M). HLA expression was evaluated with immunofluorescence, immunoperoxidase, and radioimmunoassay, using monoclonal antibodies. RB SF81 treated with retinoic acid showed a significant, persistent increase in HLA levels by 24 hours compared with controls. Thus, retinoic acid has been documented to induce the surface expression of HLA in undifferentiated retinoblastoma. Developmental parallels between embryonic retina and in vitro retinoblastoma treated with retinoic acid exist.
主要组织相容性复合体（HLA - A、B、C）I类抗原在眼细胞表面的完整表达似乎受发育调控；在16周龄胎儿眼的视网膜中检测不到HLA，但在成人视网膜中存在。培养的视网膜母细胞瘤（RB）可作为视网膜细胞发育的有用模型。SF81是体外视网膜母细胞瘤的未分化传代培养物，几乎没有自发分化的证据且缺乏HLA表达，证明了该细胞系的“未分化”特性。用视黄酸（一种已知的分化刺激剂，最佳浓度为5×10⁻⁸M）处理RB SF81细胞。使用单克隆抗体通过免疫荧光、免疫过氧化物酶和放射免疫测定法评估HLA表达。与对照组相比，用视黄酸处理的RB SF81在24小时时HLA水平显著且持续升高。因此，已证明视黄酸可诱导未分化视网膜母细胞瘤中HLA的表面表达。胚胎视网膜与用视黄酸处理的体外视网膜母细胞瘤之间存在发育平行关系。
