一种从大肠杆菌中纯化尿苷磷酸化酶的一步法。

Anal Biochem. 1983 Aug;133(1):153-6. doi: 10.1016/0003-2697(83)90236-1.

Adsorption to Matrex Gel Green A and successive elution with uridine provides a one-step procedure for the purification of uridine phosphorylase from Escherichia coli B. The homogeneous preparation of the enzyme can be obtained within a single day with an activity recovery above 80%.

用Matrex Gel Green A吸附并随后用尿苷洗脱，为从大肠杆菌B中纯化尿苷磷酸化酶提供了一种一步法。在一天内即可获得该酶的纯品制剂，活性回收率超过80%。