Andreesen R, Osterholz J, Bross K J, Schulz A, Luckenbach G A, Löhr G W
Cancer Res. 1983 Dec;43(12 Pt 1):5931-6.
Human blood-borne monocytes were cultured for up to 22 days on disposable Teflon foils. Within 8 days, these monocytes developed into mature macrophages. At various stages of differentiation, the cells were recovered from the hydrophobic membrane and were assayed for typical monocyte-macrophage enzymes and morphology, binding of monoclonal antibodies (OKM1, OKla1), Fc and transferrin receptors, phagocytic activity, lysozyme production, and ability to inhibit the growth of an allogeneic tumor target cell line (U937). A significant antitumor activity of mature macrophages was found, which developed along with the differentiation of the monocyte precursor cells. In addition, cytotoxic effector macrophages could be activated by lymphokine-rich medium and synthetic alkyl-lysophospholipids. After density gradient separation, light cells (less than 1.05 and less than 1.06 g/ml) showed enhanced cytotoxicity, whereas cells from the dense fraction (greater than 1.06 g/ml) with low base-line activity could be best activated for cytotoxicity by lymphokines. If monocyte-macrophages are involved in a natural surveillance mechanism, our results may indicate the importance of unimpaired macrophage maturation to generate effective host defense against tumor development.
人血单核细胞在一次性聚四氟乙烯箔上培养长达22天。在8天内，这些单核细胞发育成成熟的巨噬细胞。在分化的各个阶段，从疏水膜上回收细胞，并检测其典型的单核细胞-巨噬细胞酶和形态、单克隆抗体（OKM1、OKla1）的结合、Fc和转铁蛋白受体、吞噬活性、溶菌酶产生以及抑制同种异体肿瘤靶细胞系（U937）生长的能力。发现成熟巨噬细胞具有显著的抗肿瘤活性，其随着单核细胞前体细胞的分化而发展。此外，富含淋巴因子的培养基和合成烷基溶血磷脂可激活细胞毒性效应巨噬细胞。密度梯度分离后，轻细胞（小于1.05和小于1.06 g/ml）显示出增强的细胞毒性，而基线活性低的重组分细胞（大于1.06 g/ml）可被淋巴因子最佳地激活以产生细胞毒性。如果单核细胞-巨噬细胞参与自然监视机制，我们的结果可能表明未受损的巨噬细胞成熟对于产生有效的宿主抗肿瘤防御的重要性。
