Vondrejs V, Psenicka I, Kupcová L, Dostálová R, Janderová B, Bendová O
Folia Biol (Praha). 1983;29(5):372-84.
A new selection method of yeast cell hybrids, based on the application of yeast killer factors, has been developed in the model system of induced protoplast fusion of Saccharomyces cerevisiae superkiller strain T158C (alpha, his-) and different sensitive strains. The method is comparable in its efficiency to the currently used selection techniques based on the auxotrophy or respiration deficiency of the parental cells. Selection by means of a killer factor, when compared to other methods, has the following advantages: (1) Sensitivity to a killer factor is a current natural property of most of the yeast strains. (2) Killer strains of different species of yeast are available and the killer character can be easily transmitted from strain to strain. (3) Hybrid cells produce a killer factor (K+) and are resistant to it (R+). Both these properties are easily and independently testable. (4) Hybrid cells can be cured of the "killer character" by growth at elevated temperatures. Therefore the killer character can also serve as a temporary marker for the purposes of selection of hybrid cells and can be eliminated when no longer desired. On an example of interspecific fusion of strain T158C of S. cerevisiae and strain P9 of S. uvarum the procedure has been shown to be advantageous particularly for the construction of new industrial yeast strains because mutagenesis of the original industrial strain is not required.
基于酵母杀伤因子的应用，已在酿酒酵母超级杀伤菌株T158C（α，his-）与不同敏感菌株的原生质体诱导融合模型系统中开发出一种新的酵母细胞杂种选择方法。该方法的效率与目前基于亲代细胞营养缺陷或呼吸缺陷的选择技术相当。与其他方法相比，利用杀伤因子进行选择具有以下优点：（1）对杀伤因子的敏感性是大多数酵母菌株当前的天然特性。（2）有不同酵母物种的杀伤菌株，且杀伤特性可容易地从一个菌株传递到另一个菌株。（3）杂种细胞产生杀伤因子（K+）并对其具有抗性（R+）。这两种特性都易于且可独立检测。（4）杂种细胞可通过在高温下生长而消除“杀伤特性”。因此，杀伤特性也可作为选择杂种细胞的临时标记，在不再需要时可将其消除。以酿酒酵母T158C菌株与葡萄汁酵母P9菌株的种间融合为例，该方法已显示出特别有利于构建新的工业酵母菌株，因为无需对原始工业菌株进行诱变。
