Mor N
Infect Immun. 1983 Nov;42(2):802-11. doi: 10.1128/iai.42.2.802-811.1983.
Soon after more than 10(6) Mycobacterium leprae, freshly harvested from armadillo liver or harvested and 60CO irradiated, were inoculated into the hind footpads of either normal or thymectomized and irradiated (T900R) mice, the organisms were found to reside within phagosomes of polymorphonuclear and mononuclear cells. On the other hand, 7 and 8 months after 10(4) freshly harvested M. leprae were inoculated into the footpads of normal or T900R mice and the organisms had multiplied to their maximum in the normal mice, many organisms, largely intact by electron-microscopic criteria, were found to reside free in the cytoplasm of the footpad macrophages, whereas damaged organisms were contained within phagosomes. After 11 months, many intact organisms were found to lie free in the cytoplasm of the macrophages of T900R mice, whereas only damaged intraphagosomal M. leprae cells were observed in the macrophages of normal mice. Finally, a remarkably large proportion of damaged extraphagosomal M. leprae was found in T900R mice administered rifampin for 2 days in a bactericidal dosage. It appears that M. leprae multiplies free in the cytoplasm of the footpad macrophages of infected mice, whereas the M. leprae cells resident within the phagosomes of the macrophages are dead. As the result of treatment with rifampin, the organisms appeared to have been killed in their extraphagosomal location, only afterwards being incorporated into phagosomes. However, the intracellular site in which M. leprae is killed in the course of an effective immune response remains unclear.
将刚从犰狳肝脏收获的或收获后经60CO照射的10(6) 以上麻风分枝杆菌接种到正常小鼠或胸腺切除并照射（T900R）的小鼠后足垫后不久，发现这些微生物存在于多形核细胞和单核细胞的吞噬体内。另一方面，将10(4) 刚收获的麻风分枝杆菌接种到正常或T900R小鼠的足垫7至8个月后，这些微生物在正常小鼠体内已繁殖到最大数量，此时发现许多在电子显微镜标准下基本完整的微生物游离于足垫巨噬细胞的细胞质中，而受损的微生物则包含在吞噬体内。11个月后，在T900R小鼠的巨噬细胞细胞质中发现许多完整的微生物游离，而在正常小鼠的巨噬细胞中仅观察到吞噬体内受损的麻风分枝杆菌细胞。最后，在以杀菌剂量给予利福平2天的T900R小鼠中发现了大量受损的细胞外麻风分枝杆菌。似乎麻风分枝杆菌在受感染小鼠足垫巨噬细胞的细胞质中自由繁殖，而存在于巨噬细胞吞噬体内的麻风分枝杆菌细胞已死亡。由于利福平治疗的结果，这些微生物似乎在其细胞外位置被杀死，随后才被纳入吞噬体。然而，在有效免疫反应过程中麻风分枝杆菌被杀死的细胞内位置仍不清楚。
