Morris E J, McBride B C
Infect Immun. 1983 Dec;42(3):1073-80. doi: 10.1128/iai.42.3.1073-1080.1983.
A number of strains of Streptococcus sanguis were found to aggregate in nonimmune serum and in crevicular fluid. All strains which aggregated in serum also aggregated in saliva, but some strains which aggregated in saliva did not aggregate in serum. Aggregation was destroyed by treatment of serum or crevicular fluid with neuraminidase and was inhibited by gangliosides. Treatment of serum with proteases reduced aggregating activity. Adsorption of serum to hydroxyapatite did not reduce the aggregating activity. The aggregating factor was partially purified by gel filtration and polyacrylamide gel electrophoresis and was found to be an acidic glycoprotein with a molecular weight of greater than 200,000, comprised of subunits with molecular weights of approximately 100,000. It did not appear to be an immunoglobulin and could not be identified with any other serum component tested. The possible role of the aggregating factor in providing nonimmune protection against colonization of S. sanguis in the gingival crevice and blood is discussed.
发现许多血链球菌菌株能在非免疫血清和龈沟液中聚集。所有能在血清中聚集的菌株也能在唾液中聚集，但一些能在唾液中聚集的菌株不能在血清中聚集。用神经氨酸酶处理血清或龈沟液会破坏聚集，神经节苷脂会抑制聚集。用蛋白酶处理血清会降低聚集活性。血清吸附到羟基磷灰石上不会降低聚集活性。通过凝胶过滤和聚丙烯酰胺凝胶电泳对聚集因子进行了部分纯化，发现它是一种酸性糖蛋白，分子量大于200,000，由分子量约为100,000的亚基组成。它似乎不是免疫球蛋白，也无法与测试的任何其他血清成分相鉴别。讨论了聚集因子在提供针对血链球菌在龈沟和血液中定植的非免疫保护方面的可能作用。
