Tiunova N A, Kobzeva N Ia, Zaĭkina I V, Eliakova L A, Nazarova N I
Mikrobiologiia. 1983 Jul-Aug;52(4):586-90.
Different actinomyces species (25 strains) were studied and Actinomyces cellulosae 41 was found to be the most active one in its capacity to cause lysis of Saccharomyces cerevisiae intact cells as well as in the production of 1,3-beta-glucanase. The enzyme preparation containing 1,3-beta-glucanase can be obtained from the filtrate of the actinomycete cultural broth after 60 h of growth by precipitation with four volumes of ethanol. The optimal pH for the action of 1,3-beta-glucanase from A. cellulosae is 5.5. Hydrolysis of laminarin (5 mg of the substrate) yields 2.4 mg of reducing sugars or 13.3 microM (recalculated per glucose). There is a direct correlation between the amount of produced reducing sugars and the enzyme concentration up to 10 microM/ml (recalculated per glucose). The enzyme hydrolysate contains glucose and oligosaccharides with a different degree of polymerization. Therefore, the enzyme is endo-1,3-beta-glucanase. It produces less quantities of the disaccharide than those of glucose and trisaccharide. The enzyme yields only traces of glucose upon prolonged hydrolysis of p-nitrophenyl-beta-D-glucoside (PNPG) and shows a weak capacity for transglycosylation when laminarin is used as a donor and PNPG as an acceptor.
对不同的放线菌物种（25株）进行了研究，发现纤维素放线菌41在导致酿酒酵母完整细胞裂解的能力以及1,3-β-葡聚糖酶的产生方面最为活跃。含有1,3-β-葡聚糖酶的酶制剂可以在放线菌培养液生长60小时后，通过用四倍体积的乙醇沉淀从滤液中获得。纤维素放线菌的1,3-β-葡聚糖酶作用的最佳pH值为5.5。水解海带多糖（5毫克底物）可产生2.4毫克还原糖或13.3微摩尔（以葡萄糖重新计算）。在高达10微摩尔/毫升（以葡萄糖重新计算）的酶浓度范围内，产生的还原糖量与酶浓度之间存在直接相关性。酶水解产物含有葡萄糖和不同聚合度的寡糖。因此，该酶是内切-1,3-β-葡聚糖酶。它产生的二糖量比葡萄糖和三糖少。该酶在对硝基苯基-β-D-葡萄糖苷（PNPG）进行长时间水解时仅产生微量葡萄糖，并且当以海带多糖作为供体、PNPG作为受体时，其转糖基化能力较弱。
