通过加热选择性失活大肠杆菌DNA聚合酶I的3'至5'核酸外切酶活性。
Selective inactivation of the 3' to 5' exonuclease activity of Escherichia coli DNA polymerase I by heat.
作者信息
Lecomte P J, Doubleday O P
出版信息
Nucleic Acids Res. 1983 Nov 11;11(21):7505-15. doi: 10.1093/nar/11.21.7505.
Abstract
Heat selectively inactivates the 3' to 5' exonuclease activity of E. coli DNA polymerase I, resulting in reduced dNTP turnover and lower fidelity of replication of homopolymer and natural DNA templates.
摘要
热可选择性地使大肠杆菌DNA聚合酶I的3'至5'核酸外切酶活性失活，导致dNTP周转率降低以及同聚物和天然DNA模板复制的保真度降低。
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