Pedersen J, Rubenson A, Nilsson L A
Scand J Urol Nephrol. 1983;17(3):277-81. doi: 10.3109/00365598309182131.
Bilateral vasectomy was performed in 40 inbred Lewis rats. Twenty prefertile rats were operated upon at the age of 2-3 weeks and 20 adult rats at the age of 10-11 weeks. Sera were sampled 16 and 8 weeks later respectively. Circulating antisperm antibodies were demonstrated in 90% of the vasectomized rats by means of the indirect immunofluorescence technique. No significant difference between the two groups could be found. Sham-vasectomy was performed in 20 rats age 2-3 weeks and in 20 rats aged 10-11 weeks. No antisperm antibodies could be detected in sera from either of these groups 16 and 8 weeks respectively postoperatively. Also, no antisperm antibodies could be demonstrated in sera sampled from 5 control rats at the age of 2-3 weeks or in sera sampled preoperatively from the 40 rats aged 10-11 weeks. Sperm granulomas were observed in 95% of the rats vasectomized at the age of 2-3 weeks, almost, all granulomas being located in the epididymis. 95% of the rats vasectomized at the age of 10-11 weeks also developed sperm granulomas. These were located in the proximal end of the vas deferens. The difference in distribution was significant (p less than 0.001).
对40只近交系Lewis大鼠进行双侧输精管切除术。20只生育期前大鼠在2 - 3周龄时接受手术，20只成年大鼠在10 - 11周龄时接受手术。分别在术后16周和8周采集血清。通过间接免疫荧光技术在90%的输精管切除大鼠中检测到循环抗精子抗体。两组之间未发现显著差异。对20只2 - 3周龄的大鼠和20只10 - 11周龄的大鼠进行假输精管切除术。术后16周和8周，这两组大鼠的血清中均未检测到抗精子抗体。此外，在5只2 - 3周龄对照大鼠的血清中或在40只10 - 11周龄大鼠术前采集的血清中也未检测到抗精子抗体。在2 - 3周龄时接受输精管切除术的大鼠中，95%观察到精子肉芽肿，几乎所有肉芽肿都位于附睾。在10 - 11周龄时接受输精管切除术的大鼠中，95%也出现了精子肉芽肿。这些肉芽肿位于输精管近端。分布差异具有显著性（p小于0.001）。
