Shorey C D, Cleland K W
Anat Rec. 1983 Nov;207(3):523-31. doi: 10.1002/ar.1092070314.
The aim of this study was to evaluate the effects of a number of reported procedures which precede freezing on the cross sectional area of skeletal muscle fibers. Probable sources of variation were recognised as: Within blocks, same animal, same procedure. Variability in the size of muscle fibers from area-based measurements was determined for the anterior head of the biceps femoris muscle of the rat. Fibers were measured within areas selected at random from sections taken from the same block of tissue. Variance within and between sections was subsequently established. Between blocks from rats having undergone the same procedure. Variability in the size of muscle fibers measured within areas selected at random from sections taken from different blocks of tissue within the same animal. Between rats, same or equivalent procedure. Fibers were measured from randomly selected areas within sections taken from blocks of tissue derived from different animals. The muscles were subjected to the same or equivalent treatment prior to freezing. Between procedures. Restraining muscles prior to and during freezing did not significantly affect the results except the procedure involving holding the muscle between two pairs of forceps. However, the variability was high within and between sections and blocks from the same muscle. It is postulated that the variability is of technical rather than biological origin and may be the result of small focal contractions which occur along the fibers during freezing.
本研究的目的是评估一些在冷冻前进行的操作对骨骼肌纤维横截面积的影响。变异的可能来源被认为是：在同一组内，同一动物，相同操作。对大鼠股二头肌前头基于面积测量的肌纤维大小的变异性进行了测定。在从同一组织块切片中随机选择的区域内测量纤维。随后确定了切片内和切片间的方差。在接受相同操作的大鼠的组间。在从同一动物不同组织块切片中随机选择的区域内测量的肌纤维大小的变异性。在大鼠之间，相同或等效操作。从取自不同动物组织块的切片内随机选择的区域测量纤维。在冷冻前，肌肉接受相同或等效的处理。在不同操作之间。在冷冻前和冷冻过程中固定肌肉，除了用两对镊子夹住肌肉的操作外，对结果没有显著影响。然而，来自同一块肌肉的切片内和切片间以及组间的变异性都很高。据推测，这种变异性源于技术而非生物学因素，可能是冷冻过程中沿纤维发生的小范围局部收缩的结果。
