Gangadharam P R, Pratt P F
Am Rev Respir Dis. 1983 Dec;128(6):1044-7. doi: 10.1164/arrd.1983.128.6.1044.
Normal resident and BCG-activated alveolar and peritoneal macrophages from female Swiss Webster mice were compared for their ability to ingest and subsequently control the multiplication of Mycobacterium intracellulare in vitro. Resident peritoneal macrophages failed from the moment of ingestion to control the multiplication of engulfed bacilli resulting in host cell lysis, whereas activated peritoneal macrophages and both resident and activated alveolar macrophages constrained bacterial division for at least 7 days before comparable bacterial multiplication led to phagocyte death. The number of bacilli needed to lyse a macrophage was impossible to determine precisely because viable macrophages commonly contained several hundred mycobacteria. Minimal intracellular bacterial generation times were 20 h for each macrophage type. Differences in the rates of bacterial phagocytosis between both macrophage types, either resident or activated, are intrinsic properties of the macrophages and were not induced by the mycobacteria, because the same patterns of particle ingestion were observed after exposure to latex microspheres.
对来自雌性瑞士韦伯斯特小鼠的正常常驻型以及卡介苗激活型肺泡巨噬细胞和腹腔巨噬细胞在体外摄取并随后控制胞内分枝杆菌增殖的能力进行了比较。常驻腹腔巨噬细胞从摄取之时起就无法控制被吞噬杆菌的增殖，导致宿主细胞裂解，而激活的腹腔巨噬细胞以及常驻和激活的肺泡巨噬细胞在相当的细菌增殖导致吞噬细胞死亡之前，将细菌分裂抑制了至少7天。由于活的巨噬细胞通常含有数百个分枝杆菌，因此无法精确确定裂解一个巨噬细胞所需的杆菌数量。每种巨噬细胞类型的最小细胞内细菌世代时间均为20小时。常驻或激活的两种巨噬细胞类型之间细菌吞噬速率的差异是巨噬细胞的固有特性，并非由分枝杆菌诱导，因为在接触乳胶微球后观察到相同的颗粒摄取模式。
