新月体性肾小球肾炎中细胞群的分析

Analysis of cell populations in crescentic glomerulonephritis.

作者信息

Sarno E N, Gattass C R, Alvarenga F B, Vieira L M, Dos-Santos S F

出版信息

Braz J Med Biol Res. 1983 Oct;16(3):227-33.

Abstract

The cell types present in the crescents were studied in 5 human patients with crescentic glomerulonephritis: two cases of systemic lupus erythematosus, one case of hemolytic uremic syndrome and two cases of rapidly progressive glomerulonephritis. Frozen sections of renal biopsies were studied by immunofluorescence, using murine monoclonal antibodies (orthoclones) against specific antigens on the membrane of human peripheral blood cells, and by histochemical methods. Monocytes (OKM1+, OKIa+ cells) but no lymphocytes (OKT+ cells), were detected in the crescentic glomeruli. Subsets of T lymphocytes (inducer-helper and cytotoxic-suppressor) were detected in the interstitium. Non-specific esterase-positive cells were observed in the glomeruli and in small numbers in the crescents. Fibrinogen deposits were present in the crescents of four of the five cases studied. No immunoglobulins (IgG, IgM, IgA) or complement (C1q, C3) deposits were detected in the crescents. Fibrinogen, immunoglobulins and complement were present in the glomerular tufts.

摘要

对5例新月体性肾小球肾炎患者的新月体中的细胞类型进行了研究：2例系统性红斑狼疮、1例溶血性尿毒症综合征和2例快速进行性肾小球肾炎。通过免疫荧光法，使用针对人外周血细胞表面特定抗原的鼠单克隆抗体（orthoclones），以及组织化学方法，对肾活检的冰冻切片进行研究。在新月体性肾小球中检测到单核细胞（OKM1 +、OKIa +细胞），但未检测到淋巴细胞（OKT +细胞）。在间质中检测到T淋巴细胞亚群（诱导-辅助细胞和细胞毒性-抑制细胞）。在肾小球中观察到非特异性酯酶阳性细胞，在新月体中数量较少。在研究的5例患者中的4例新月体中存在纤维蛋白原沉积。在新月体中未检测到免疫球蛋白（IgG、IgM、IgA）或补体（C1q、C3）沉积。纤维蛋白原、免疫球蛋白和补体存在于肾小球丛中。