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化学诱变和致癌作用机制：dGTP的O6-鸟嘌呤位置甲基化对DNA复制的影响。

Mechanisms of chemical mutagenesis and carcinogenesis: effects on DNA replication of methylation at the O6-guanine position of dGTP.

作者信息

Toorchen D, Topal M D

出版信息

Carcinogenesis. 1983 Dec;4(12):1591-7. doi: 10.1093/carcin/4.12.1591.

DOI:10.1093/carcin/4.12.1591
PMID:6360407
Abstract

The incorporation of O6-methyl-dGTP during DNA replication in vitro by 'Klenow' E. coli pol I was determined. O6-Methyl-dGTP was found to: (i) incorporate opposite T and C template residues, with a greater than 20-fold preference for T, and (ii) arrest DNA synthesis when incorporated in place of dATP at all but pyrimidine-rich growing-strand sequences. The significance of O6-methyl-dGTP incorporation during DNA biosynthesis in vivo for mutagenesis and carcinogenesis is discussed.

摘要

测定了体外DNA复制过程中“Klenow”大肠杆菌DNA聚合酶I对O6-甲基-dGTP的掺入情况。发现O6-甲基-dGTP：（i）与T和C模板残基相对掺入，对T的偏好性大于20倍，以及（ii）当在除富含嘧啶的生长链序列外的所有位置取代dATP掺入时会阻止DNA合成。讨论了体内DNA生物合成过程中O6-甲基-dGTP掺入对诱变和致癌作用的意义。

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