通过二维聚丙烯酰胺凝胶电泳展示蛋白酶同工酶
Demonstration of protease isoenzymes by two-dimensional polyacrylamide electrophoresis.
作者信息
Sinha P K, Gossrau R
出版信息
Histochemistry. 1983;79(3):483-6. doi: 10.1007/BF00491782.
PMID:6360965
Abstract
A two-dimensional polyacrylamide electrophoresis system is described for the detection of protease isoenzymes using 4-methoxy-2-naphthylamine derivates as substrates. With this technique is possible to detect isoenzymes differing in molecular weight by separation according to size and shape. This is not possible by the use of isoelectric focusing alone.
摘要
描述了一种二维聚丙烯酰胺电泳系统，用于以4-甲氧基-2-萘胺衍生物作为底物检测蛋白酶同工酶。通过这种技术，根据大小和形状进行分离，可以检测分子量不同的同工酶。仅使用等电聚焦则无法做到这一点。
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