Fucci L, Cirotto C, Tomei L, Geraci G
J Embryol Exp Morphol. 1983 Oct;77:153-65.
The synthesis of globins in the chick embryo before the onset of circulation has been studied in situ by specific immunofluorescence labelling of embryonic sections and by labelling newly synthesized proteins in ovo and in vitro in embryonic explants with [3H]leucine. The presence of major primitive haemoglobins is observed by 28 h of incubation. The minor primitive haemoglobins become detectable by immunofluorescence after 40 h of development, shortly before the onset of circulation. 3H-labelling shows that one definitive alpha chain is synthesized, though in low concentration, from the initial globin detection. The other definitive alpha chain is observed in embryos of at least 40 h of development. The relative concentration of the two definitive alpha chains changes rapidly with development indicating a specific mechanism of regulation. An erythropoietic site is observed in the wall of the dorsal aorta in embryos of about 45-50 h of development. From the initial detection, those cells contain all four primitive embryonic haemoglobins, in contrast to what is observed for the cells of the blood islands.
通过对胚胎切片进行特异性免疫荧光标记，以及用[3H]亮氨酸在胚胎外植体中对胚胎在体内和体外新合成的蛋白质进行标记，对鸡胚循环开始前珠蛋白的合成进行了原位研究。孵育28小时后可观察到主要原始血红蛋白的存在。发育40小时后，即在循环开始前不久，通过免疫荧光可检测到次要原始血红蛋白。3H标记显示，从最初检测到珠蛋白开始，就合成了一条确定的α链，尽管浓度较低。在至少发育40小时的胚胎中观察到另一条确定的α链。两条确定的α链的相对浓度随发育迅速变化，表明存在特定的调节机制。在发育约45 - 50小时的胚胎的背主动脉壁中观察到一个造血部位。从最初检测开始，这些细胞就含有所有四种原始胚胎血红蛋白，这与血岛细胞的情况不同。
