Zagris N
J Embryol Exp Morphol. 1980 Aug;58:209-16.
Morphologically distinct erythroid cell types characteristic of the primitive and the definitive erythroid cell lines, and embryonic and adult haemoglobins are produced when the unincubated chick blastoderm is cultured ventral side down on a filter raft to inhibit morphogenetic movements and subsequent primitive-streak formation mechanically in serum-free minimal essential medium. The primitive and definitive erythroid cell populations appear consecutively in culture even though there is no axis formation nor apparent morphogenesis. The information to produce both the early and late haemoglobins and erythroid cell types is independent of axis formation and of specific extra-embryonic influences, such as progressive induction exerted by the yolk mass.
当未孵化的鸡胚盘腹面朝下培养在滤膜筏上，以在无血清的基本培养基中机械抑制形态发生运动和随后的原条形成时，会产生原始和定型红细胞系特有的形态学上不同的红细胞类型，以及胚胎型和成人型血红蛋白。即使没有轴的形成也没有明显的形态发生，原始和定型红细胞群体仍会在培养物中相继出现。产生早期和晚期血红蛋白以及红细胞类型的信息独立于轴的形成以及特定的胚外影响，例如卵黄物质施加的渐进诱导。
