肺炎克雷伯菌固氮基因在粪产碱菌（一种与水稻根相关的固氮细菌）中的转移与表达。

Transfer and expression of Klebsiella nif genes in Alcaligenes faecalis, a nitrogen-fixing bacterium associated with rice root.

作者信息

Chen J M, Ye Z H

出版信息

Plasmid. 1983 Nov;10(3):290-2. doi: 10.1016/0147-619x(83)90043-4.

Abstract

Plasmid pRD1 carrying Klebsiella nif genes was found to be transferable by conjugation from Escherichia coli JC5466 (pRD1) to Alcaligenes faecalis A-15 at a frequency of 5 X 10(-4). Nitrogenase activity of four A-15 (pRD1) strains tested was found to be higher than that of their parent A-15, as determined by the acetylene reduction assay. A-15-1 was a Nif- mutant derived from A-15. After mating with JC5466 (pRD1), the nitrogenase activity was restored. PRD1 was stable in A. faecalis and could be transferred to E. coli JC5466-1 by conjugation. The fact that Klebsiella nif genes carried in pRD1 can be expressed in A. faecalis makes it possible to use pRD1 as a tool for genetic analysis and genetic engineering of the nitrogen fixation system in A. faecalis.

摘要

携带肺炎克雷伯菌固氮基因的质粒pRD1可通过接合作用从大肠杆菌JC5466（pRD1）转移至粪产碱菌A - 15，转移频率为5×10⁻⁴。通过乙炔还原法测定发现，所测试的4株粪产碱菌A - 15（pRD1）菌株的固氮酶活性高于其亲本A - 15。A - 15 - 1是源自A - 15的固氮基因缺失突变体。与JC5466（pRD1）接合后，其固氮酶活性得以恢复。pRD1在粪产碱菌中稳定，并且可通过接合作用转移至大肠杆菌JC5466 - 1。pRD1携带的肺炎克雷伯菌固氮基因可在粪产碱菌中表达，这一事实使得pRD1有可能用作粪产碱菌固氮系统遗传分析和基因工程的工具。