Lehtinen H, Mäntsälä P
Antonie Van Leeuwenhoek. 1981 Dec;47(5):405-10. doi: 10.1007/BF00426002.
The plasmid pRD1 containing the nif genes from Klebsiella pneumoniae was transferred by conjugation from Escherichia coli to Pseudomonas putida isolated from the tundra soil. 6-Cyanopurine, acetylene reduction and immunological tests showed that the nif genes were not expressed in P. putida. Existence of the nif genes in P. putida transconjugants was detected by transferring them to E. coli C600, which does not fix nitrogen. Existence of the nif genes in E. coli C600 transconjugants was detected immunologically and by acetylene reduction tests.
含有肺炎克雷伯氏菌固氮基因的质粒pRD1通过接合作用从大肠杆菌转移至从冻原土壤中分离出的恶臭假单胞菌。6-氰基嘌呤、乙炔还原和免疫学试验表明，固氮基因在恶臭假单胞菌中未表达。通过将恶臭假单胞菌接合子转移至不固氮的大肠杆菌C600，检测到恶臭假单胞菌接合子中固氮基因的存在。通过免疫学方法和乙炔还原试验检测了大肠杆菌C600接合子中固氮基因的存在。
