Bockrath R, Wolff L, Person S
Radiat Res. 1983 Dec;96(3):635-40.
The mutagenic local effect of tritium decay at the 5 position of cytosine in DNA of Escherichia coli was determined in wild-type and in ung strains defective in uracil-DNA glycosylase. In the absence of this in vivo activity any genetic consequences of uracil residues formed in DNA should be enhanced. However, the mutation frequency response was no greater in the mutant strain than in the wild type. This finding is inconsistent with the earlier suggestion that efficient production of C to T transitions by the local effect of [5-3H]cytosine decay results from the formation of uracil in cellular DNA. Some other intermediate should be considered, one that is not a substrate for uracil-DNA glycosylase.
在野生型和尿嘧啶-DNA糖基化酶缺陷的ung菌株中，测定了氚衰变对大肠杆菌DNA中胞嘧啶5位的诱变局部效应。在缺乏这种体内活性的情况下，DNA中形成的尿嘧啶残基的任何遗传后果都应增强。然而，突变频率反应在突变菌株中并不比野生型更大。这一发现与早期的观点不一致，即[5-3H]胞嘧啶衰变的局部效应导致C到T转换的有效产生是由于细胞DNA中尿嘧啶的形成。应该考虑其他一些中间体，一种不是尿嘧啶-DNA糖基化酶底物的中间体。
