Boeynaems J M, Galand N
Prostaglandins. 1983 Oct;26(4):531-44. doi: 10.1016/0090-6980(83)90191-0.
The production of prostacyclin by rings of rabbit aorta was assessed by the radioimmunoassay of 6-keto-PGF1 alpha. In steady-state conditions, the rings released 11 ng 6-K-PGF1 alpha per 100 mg tissue in 30 min. Acetylcholine increased this output: a significant effect was detected at 1 microM and at 10 microM the amplitude of stimulation was 10-fold. The production of PGE2 and PGF2 alpha was also increased, but to a lesser extent. The stimulatory action of acetylcholine was mimicked by carbamylcholine and inhibited by atropine; it was abolished in a calcium-free medium. Dog and rat aorta also produced more 6-K-PGF1 alpha in response to cholinergic agonists. A short rubbing of the intimal surface of the aorta removed the layer of endothelial cells and completely abolished the cholinergic effect. It is concluded that in the aorta, cholinergic agonists, acting on a muscarinic receptor, stimulate the production of prostacyclin by endothelial cells.
通过对6-酮-前列腺素F1α进行放射免疫测定来评估兔主动脉环中前列环素的生成。在稳态条件下，主动脉环在30分钟内每100毫克组织释放11纳克6-酮-前列腺素F1α。乙酰胆碱增加了这种释放量：在1微摩尔时检测到显著作用，在10微摩尔时刺激幅度为10倍。前列腺素E2和前列腺素F2α的生成也增加了，但程度较小。卡巴胆碱模拟了乙酰胆碱的刺激作用，阿托品则抑制了该作用；在无钙培养基中该作用消失。犬和大鼠的主动脉对胆碱能激动剂也产生更多的6-酮-前列腺素F1α。对主动脉内膜表面进行短暂摩擦可去除内皮细胞层，并完全消除胆碱能效应。得出的结论是，在主动脉中，胆碱能激动剂作用于毒蕈碱受体，刺激内皮细胞生成前列环素。
