Khoury E L, Bottazzo G F, Roitt I M
J Exp Med. 1984 Feb 1;159(2):577-91. doi: 10.1084/jem.159.2.577.
We have shown that thyroid monolayers derived from the glands of patients with autoimmune thyroid disease have immunoglobulin (Ig) bound to their surface. This appears to have been deposited in vivo rather than during preparation of the monolayers, a view supported by our finding of such deposits on the apical margin of follicular cells in sections cut from these glands and stained with conjugated anti-immunoglobulin. It is likely that these deposits represent specific binding of so-called "microsomal" autoantibodies to the surface of the thyroid cells in vivo since staining of partially disrupted follicles ("half-melons") with Hashimoto serum containing microsomal autoantibodies in the indirect immunofluorescence (IFL) test, localized the antigen on the apical surface of the cells lining the follicular cavity. Thus, paradoxically, although the antigen is relatively inaccessible, autoantibodies do reach and combine with the thyroid surface in vivo and may therefore play a role in pathogenesis.
我们已经证明，源自自身免疫性甲状腺疾病患者腺体的甲状腺单层细胞表面结合有免疫球蛋白（Ig）。这似乎是在体内沉积的，而非在单层细胞制备过程中沉积，我们在这些腺体切下的切片中，用偶联抗免疫球蛋白染色后，在滤泡细胞顶端边缘发现此类沉积物，这一发现支持了这一观点。这些沉积物很可能代表所谓的“微粒体”自身抗体在体内与甲状腺细胞表面的特异性结合，因为在间接免疫荧光（IFL）试验中，用含有微粒体自身抗体的桥本血清对部分破坏的滤泡（“半个瓜”）进行染色时，抗原定位于滤泡腔内衬细胞的顶端表面。因此，矛盾的是，尽管抗原相对难以接近，但自身抗体确实能在体内到达并与甲状腺表面结合，因此可能在发病机制中起作用。
