Czarnocka B, Ruf J, Ferrand M, Lissitzky S, Carayon P
J Endocrinol Invest. 1986 Apr;9(2):135-8. doi: 10.1007/BF03348084.
Among the several antigens involved in autoimmune thyroid diseases, the microsomal antigen has been recently identified as the human thyroid peroxidase (TPO). The availability of highly purified TPO and anti-TPO monoclonal antibody (mAb) allowed us to study in more details anti-TPO autoimmune antibodies (aAb) and their relationship with anti-microsomal aAb. Only sera with anti-microsomal aAb, as assayed by passive hemagglutination, highly immunoprecipitated purified TPO; anti-thyroglobulin aAb did not contribute to this effect. IgG binding to TPO and inhibition of mAb binding to TPO were also observed using anti-microsomal positive and anti-thyroglobulin negative sera. The correlation between anti-TPO and anti-microsomal aAb titers was found significant (p less than 0.02). These data suggest that anti-microsomal and anti-TPO immunoreactivity are presented by the same aAb. It could not be ruled out, however, that some of the anti-microsomal aAb detected by passive hemagglutination could be directed against antigens different from TPO. The use of highly purified TPO and anti-TPO mAb would allow large scale studies of anti-TPO aAb, necessary for investigating their pathological significance in thyroid disorders.
在几种与自身免疫性甲状腺疾病相关的抗原中，微粒体抗原最近被鉴定为人甲状腺过氧化物酶（TPO）。高纯度TPO和抗TPO单克隆抗体（mAb）的可得性使我们能够更详细地研究抗TPO自身抗体（aAb）及其与抗微粒体aAb的关系。通过被动血凝试验检测，只有含有抗微粒体aAb的血清能高度免疫沉淀纯化的TPO；抗甲状腺球蛋白aAb对此效应无作用。使用抗微粒体阳性和抗甲状腺球蛋白阴性血清也观察到了IgG与TPO的结合以及mAb与TPO结合的抑制。发现抗TPO和抗微粒体aAb滴度之间的相关性显著（p小于0.02）。这些数据表明抗微粒体和抗TPO免疫反应性由相同的aAb呈现。然而，不能排除通过被动血凝试验检测到的一些抗微粒体aAb可能针对与TPO不同的抗原。高纯度TPO和抗TPO mAb的使用将允许对抗TPO aAb进行大规模研究，这对于研究它们在甲状腺疾病中的病理意义是必要的。
