Scherer-Singler U, Vincent S R, Kimura H, McGeer E G
J Neurosci Methods. 1983 Nov;9(3):229-34. doi: 10.1016/0165-0270(83)90085-7.
A simple enzyme histochemical technique is described that detects various distinct populations of neurons in the brain. These neurons contain an extremely high activity of an endogenous enzyme, NADPH-diaphorase, that can reduce the dye nitro blue tetrazolium to a bright blue reaction product. Some of the major groups of neurons detected by this technique occur scattered throughout the neocortex, in the striatum and in the laterodorsal tegmental nucleus. The soma, dendritic trees and fiber networks of the positive neurons are stained in their entirety. Thus this simple, reliable technique can be used to obtain a Golgi-like image of particular groups of neurons in various regions of the brain.
本文描述了一种简单的酶组织化学技术，该技术可检测大脑中各种不同的神经元群体。这些神经元含有一种内源性酶——NADPH-黄递酶，其活性极高，能将染料硝基蓝四氮唑还原为亮蓝色反应产物。通过该技术检测到的一些主要神经元群分散在整个新皮层、纹状体和外侧背侧被盖核中。阳性神经元的胞体、树突和纤维网络均被完整染色。因此，这种简单可靠的技术可用于获得大脑不同区域特定神经元群的类似高尔基染色的图像。
