Vincent S R, Satoh K, Armstrong D M, Fibiger H C
Neurosci Lett. 1983 Dec 23;43(1):31-6. doi: 10.1016/0304-3940(83)90124-6.
Choline acetyltransferase immunohistochemistry has identified a large group of cholinergic neurons in the pontine tegmentum. By combined immunohistochemical and enzyme histochemical studies this particular cholinergic cell group was found to contain an enzyme, NADPH-diaphorase, that can be visualized histochemically. Thus NADPH-diaphorase histochemistry provides a simple, reliable method to selectively stain the cholinergic neurons of the brainstem reticular formation. The resolution obtained by this novel histochemical technique is similar to that found with the Golgi stain, and it should therefore be of great value in morphological studies of this cholinergic cell group.
胆碱乙酰转移酶免疫组织化学已在脑桥被盖中鉴定出一大群胆碱能神经元。通过免疫组织化学和酶组织化学的联合研究发现，这个特定的胆碱能细胞群含有一种酶，即还原型辅酶Ⅱ黄递酶，可通过组织化学方法使其可视化。因此，还原型辅酶Ⅱ黄递酶组织化学提供了一种简单、可靠的方法来选择性地染色脑干网状结构中的胆碱能神经元。这种新型组织化学技术所获得的分辨率与高尔基染色法相似，因此在对这个胆碱能细胞群的形态学研究中应该具有很大的价值。
