Kung H, Bekesi E, Guterman S K, Gray J E, Traub L, Calhoun D H
Mol Gen Genet. 1984;193(2):210-3. doi: 10.1007/BF00330669.
It has previously been proposed, based on indirect evidence, that the Rho protein may control the expression of the rho gene. Using an in vitro system for the transcription and translation of the rho gene cloned into plasmid pBR322, we tested this hypothesis directly by monitoring the effect in vitro of excess or limiting Rho protein. The addition of purified Rho protein suppresses Rho synthesis in vitro. The addition of antibody to Rho specifically stimulates Rho synthesis in vitro. The stimulation of Rho factor synthesis by antibody to Rho is reversed by Rho protein. Rho factor purified from a strain with a mutationally altered rho gene (rho-115) does not suppress Rho synthesis in vitro. These results provide convincing evidence that the rho gene is subject to autoregulation.
以前曾基于间接证据提出，Rho蛋白可能控制rho基因的表达。利用克隆到质粒pBR322中的rho基因的体外转录和翻译系统，我们通过监测过量或有限的Rho蛋白在体外的作用直接检验了这一假设。添加纯化的Rho蛋白可在体外抑制Rho的合成。添加抗Rho抗体可在体外特异性刺激Rho的合成。抗Rho抗体对Rho因子合成的刺激作用可被Rho蛋白逆转。从具有突变改变的rho基因（rho-115）的菌株中纯化的Rho因子在体外不抑制Rho的合成。这些结果提供了令人信服的证据，表明rho基因受到自动调节。
