Modification of gas-chromatographic method for blood chlorpropamide determination and evaluation of its use for clinical and pharmacological purposes.

A gas-chromatographic method for isolation and determination of blood chlorpropamide, described originally in literature [10], was modified and its usefulness for clinical and pharmacological purposes evaluated by testing fluctuations in blood chlorpropamide concentration with time in healthy subjects (control group) and patients with type II diabetes mellitus, in relation to glycemia and insulinemia (IRI). In this respect the effects of a single dose and of prolonged chlorpropamide therapy were studied. Large individual variability in the time-related concentration curves and no statistically significant correlation between chlorpropamide, insulin and glycemia were noted.