Zuckerman M J, Taffet S L, Kadish A S, Das K M
Am J Gastroenterol. 1984 Feb;79(2):104-8.
Indirect immunofluorescence studies were performed to determine whether the antigenic recognition by Crohn's disease (CD) sera of lymphoid tissue from nude (nu/nu) mice injected with CD filtrate is related to cell wall defective pseudomonas-like bacterial revertant forms (CWD-RF). Antisera raised in rabbits against two CWD-RF isolates from CD tissues did not stain lymphomas or hyperplastic lymph nodes produced by CD filtrates, although these tissues demonstrated positive immunofluorescence with CD sera. Pre-absorption of reactive CD sera with CWD-RF did not block this immunofluorescence. Formalinized suspensions of CWD-RF were injected into 36 nu/nu, 12 nu/+, and 31 conventional mice. Thirty other mice were fed suspensions of bacteria. Several nu/nu injected with CWD-RF developed lymphoma (n = 2) and plasma cell hyperplasia (n = 5), none of which reacted with CD sera. Mice fed bacteria did not show intestinal pathology. These studies demonstrate that CWD-RF are not directly related to lymphomagenesis in nu/nu induced by CD filtrates and that different CD serum antibodies are involved in recognition of CWD-RF and lymphoid tissues from nu/nu previously injected with CD filtrates.
进行间接免疫荧光研究，以确定克罗恩病（CD）血清对注射了CD滤液的裸（nu/nu）小鼠淋巴组织的抗原识别是否与细胞壁缺陷型假单胞菌样细菌回复型（CWD-RF）有关。用来自CD组织的两种CWD-RF分离株免疫兔制备的抗血清，不能使由CD滤液产生的淋巴瘤或增生性淋巴结染色，尽管这些组织用CD血清显示出阳性免疫荧光。用CWD-RF对反应性CD血清进行预吸收并不能阻断这种免疫荧光。将甲醛固定的CWD-RF悬液注射到36只nu/nu小鼠、12只nu/+小鼠和31只常规小鼠体内。另外30只小鼠喂食细菌悬液。几只注射了CWD-RF的nu/nu小鼠发生了淋巴瘤（n = 2）和浆细胞增生（n = 5），但均未与CD血清发生反应。喂食细菌的小鼠未出现肠道病变。这些研究表明，CWD-RF与CD滤液诱导的nu/nu小鼠淋巴瘤发生没有直接关系，并且不同的CD血清抗体参与了对CWD-RF和先前注射过CD滤液的nu/nu小鼠淋巴组织的识别。
