Courcol R, Martin G
Ann Biol Clin (Paris). 1983;41(5):331-5.
The authors evaluate an automatic microplate reader (Auto Reader Dynatech) and its application software. 291 strains of Enterobacteria belonging to 28 species were identified and 1,856 MIC were determined. The identifications made with the MIC 2000 tube were correct in terms of genus and species in 270 strains (92.8%) and in terms of genus in 274 strains (94.1%). The automated determination of the MIC was in complete agreement with visual reading in 90.6% of cases and with a deviation of +/- 1 dilution in 99.6% of cases. The programme was improved by the authors. This system is a new approach to automatization in microbiology.
作者评估了一种自动酶标仪(Auto Reader Dynatech)及其应用软件。对属于28个物种的291株肠杆菌进行了鉴定,并测定了1856个最低抑菌浓度(MIC)。使用MIC 2000试管进行的鉴定在属和种方面,270株(92.8%)正确,在属方面274株(94.1%)正确。MIC的自动测定在90.6%的情况下与目视读数完全一致,在99.6%的情况下偏差为+/-1个稀释度。作者对该程序进行了改进。该系统是微生物学自动化的一种新方法。