Köhler C, Chan-Palay V
Anat Embryol (Berl). 1983;167(1):53-65. doi: 10.1007/BF00304600.
The distribution of gamma aminobutyric acid (GABA)-containing neurons and nerve terminals was examined in the rat septal area by using specific antibodies to the enzyme glutamic acid decarboxylase (GAD) in combination with the avidin-biotin immunoperoxidase method. Whereas only a few GAD positive neurons were present in the septum of normal rats, the septal area of rats treated with colchicine, an inhibitor of fast axonal transport, showed numerous GAD-immunoreactive neurons. These neurons were evenly filled with GAD-immunoreactive material throughout the cytoplasm of the soma and proximal parts of the dendrites. Although GAD-positive neurons were present in most parts of the septal area, their density differed greatly in the different septal subnuclei. Both the diagonal band of Broca (vertical and horizontal parts) and the lateral septum were rich in GAD positive cell bodies, whereas the medial septal nucleus and the intermediate parts of the lateral septum contained relatively few. Within the lateral septum itself a larger number of labeled cell bodies was present in its ventral subdivision. The anterior hippocampal rudiment (taenia tecta) contained numerous GAD-positive neurons, while the septal component of the island of Calleja (insula magna) was devoid of them. GAD-immuno-positive neurons found within the septum ranged from small (15 microns) to large (30-35 micron). They were round or multipolar in the diagonal band, medium-sized multipolar in the lateral septum, and pyramidal, round or fusiform in the anterior hippocampal rudiment. GAD-immunoreactive nerve terminals are present in most subdivisions of the septal nuclei, with the exception of myelinated fiber tracts, and throughout all rostrocaudal levels of the septum. However, the density of the innervation is not the same within all individual nuclei. The lateral septum (dorsal and ventral parts) contained high density innervation but the diagonal band of Broca had a lower density of GAD-positive terminals. The lateral border of the islands of Calleja was rich in thick GAD-positive processes that appeared to be continuous with GAD-immunoreactive processes of the substantia inominata. The inner portion of the molecular layer adjacent to the granule cells of the anterior hippocampal rudiment contained a rich GAD-positive terminal field.
运用针对谷氨酸脱羧酶（GAD）的特异性抗体并结合抗生物素蛋白-生物素免疫过氧化物酶法，研究了大鼠隔区中含γ-氨基丁酸（GABA）的神经元和神经终末的分布情况。正常大鼠的隔区中仅存在少数GAD阳性神经元，而用秋水仙碱（一种快速轴突运输抑制剂）处理的大鼠的隔区，可见大量GAD免疫反应阳性神经元。这些神经元的整个胞体细胞质和树突近端均均匀充满GAD免疫反应物质。尽管GAD阳性神经元存在于隔区的大部分区域，但它们在不同的隔区亚核中的密度差异很大。布罗卡斜角带（垂直部和水平部）和外侧隔核富含GAD阳性细胞体，而内侧隔核和外侧隔核的中间部分则相对较少。在外侧隔核本身，其腹侧亚区存在较多标记细胞体。前海马原基（带 tecta）含有大量GAD阳性神经元，而卡耶哈岛（insula magna）的隔区成分则没有。隔区内发现的GAD免疫阳性神经元大小不一，小的（15微米）到大的（30 - 35微米）都有。它们在斜角带呈圆形或多极形，在外侧隔核呈中等大小多极形，在前海马原基呈锥形、圆形或梭形。GAD免疫反应阳性神经终末存在于隔核的大多数亚区，有髓纤维束除外，并且在隔区的所有 rostrocaudal 水平均有分布。然而，在所有单个核内的神经支配密度并不相同。外侧隔核（背侧和腹侧部分）神经支配密度高，但布罗卡斜角带的GAD阳性终末密度较低。卡耶哈岛的外侧边界富含粗大的GAD阳性突起，这些突起似乎与无名质的GAD免疫反应阳性突起相连。与前海马原基颗粒细胞相邻的分子层内部含有丰富的GAD阳性终末区。
