Richardson M D, Smith H
Mycopathologia. 1983 Nov 21;83(2):97-102. doi: 10.1007/BF00436889.
Phagocyte monolayers provided a simple method of following ultrastructural events associated with phagocytosis and intracellular killing of Candida albicans. Preformed monolayers of mouse polymorphonuclear (PMN) phagocytes attached to glass coverslips were incubated with blastospore phase C. albicans and then examined by scanning and transmission electron microscopy. Scanning electron microscopy revealed phagocytosis of C. albicans by mouse phagocytes. Ingestion of the organism was facilitated by the production of lamellipodia by the phagocytes. Transmission electron microscopy revealed complete phagocytosis of C. albicans and the fusion of lysosomal granules with loose and tight phagosomes. Ingested C. albicans remained structurally intact after 2 hr incubation in blastospore-free medium. However, cytoplasmic alterations were clearly evident, with a patchy loss of electron density. Alterations of the blastospore cell wall were also observed, with complete disruption of the plasma membrane but the wall remaining morphologically intact.
吞噬细胞单层提供了一种简单的方法来追踪与白色念珠菌吞噬作用和细胞内杀伤相关的超微结构事件。将预先形成的附着在玻璃盖玻片上的小鼠多形核（PMN）吞噬细胞单层与白色念珠菌芽生孢子期培养物一起孵育，然后通过扫描电子显微镜和透射电子显微镜进行检查。扫描电子显微镜显示小鼠吞噬细胞对白色念珠菌的吞噬作用。吞噬细胞产生的片状伪足促进了该生物体的摄取。透射电子显微镜显示白色念珠菌被完全吞噬，溶酶体颗粒与松散和紧密的吞噬体融合。在无芽生孢子的培养基中孵育2小时后，摄入的白色念珠菌在结构上保持完整。然而，细胞质改变明显可见，电子密度有片状丧失。还观察到芽生孢子细胞壁的改变，质膜完全破坏，但细胞壁在形态上保持完整。
