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[用于肠炎沙门氏菌(盖尔特纳氏菌)鉴别的发酵与产大肠杆菌素特性]

[Fermentation and colicinogeny for differentiation of Salmonella enteritidis (Gaertner's bacterium)].

作者信息

Wuthe H H, Brandecker D

出版信息

Zentralbl Bakteriol Mikrobiol Hyg B. 1983 Apr;177(3-4):359-64.

PMID:6367305
Abstract

Study material of 435 strains of Salmonella enteritidis which were predominantly cultivated from human specimens in Schleswig-Holstein in the years from 1968 to 1978, was used for investigating the biochemical differentiation by the fermentation of arabinose, dulcitol, rhamnose and glycerol (method after Kauffmann); moreover, the decarboxylation of lysine and the gas formation from glucose as well as the colicinogenicity (indicator Escherichia coli K 12 ROW) were tested. The 4 classical biotypes showed the following rates of incidence (in %): Jena 77.2; Danysz 2.3; Chaco 1.6; Essen 9.0; 9.9 per cent of all isolates did not permit classification, among them there were 19 (4.4%) strains of unknown rhamnose-negative biotypes some of which formed no gas from glucose. Four cultures (1 Jena strain, 3 Danysz strains) were LDC-negative. The colicinogenicity was identified in 20 isolates, among them 18 strains alone in a single outbreak. The analyses revealed that the fermentation properties were constant within outbreaks while the colicinogenicity may get lost in some strains within an outbreak.

摘要

研究材料取自1968年至1978年间在石勒苏益格-荷尔斯泰因州主要从人类标本中培养出的435株肠炎沙门氏菌,用于通过阿拉伯糖、卫矛醇、鼠李糖和甘油发酵研究其生化分化(考夫曼方法);此外,还测试了赖氨酸脱羧、葡萄糖产气以及产大肠杆菌素性(指示菌大肠杆菌K12 ROW)。4种典型生物型的发生率如下(%):耶拿型77.2;达尼斯型2.3;查科型1.6;埃森型9.0;9.9%的分离株无法分类,其中有19株(4.4%)为未知的鼠李糖阴性生物型菌株,部分菌株不产生葡萄糖产气。4株培养物(1株耶拿型菌株,3株达尼斯型菌株)为赖氨酸脱羧酶阴性。在20株分离株中鉴定出了产大肠杆菌素性,其中18株仅在一次暴发中出现。分析表明,发酵特性在暴发中是恒定的,而产大肠杆菌素性在一次暴发中的某些菌株中可能会消失。

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