Skrzypulec Z A
Zentralbl Gynakol. 1984;106(1):46-54.
Due to the elucidation of transplantation phenomena of the human allogenic egg, as yet unknown, the antigenicity of each trophoblastic cells was estimated using a cell culture technique introducing the immunofluorescence method. It was studied by means of an immunological model like the fetomaternal incompatibility in the classic AB0-blood system. The in vitro cultured placenta cells (epithelials cells of the chorionic villi, the Langhanscells, the Hofbauercells and also their's daughter cells) were investigated concerning the presence of A- and B-bloodgroup antigens, whereas the direct and indirect immunofluorescence method was introduced. It was confirmed that the chorionic villi epithelial cells are always antigen-less ones. The Langhanscells possess the A- and B-transplantation antigens within their nuclei membranes only. But in Hofbauercells the presence of the antigens could be found within the cell membrane, within the nuclei membrane and also the cytoplasma of the daughter cells. The lack of transplantation antigens in the epithelial layer of the chorionic villi enable as well the placentation and further development of the human allogenic egg.
由于对人类同种异体卵子移植现象的阐明（这一现象此前尚不为人知），使用引入免疫荧光法的细胞培养技术对每个滋养层细胞的抗原性进行了评估。通过一种类似于经典ABO血型系统中母胎不相容性的免疫模型进行了研究。对体外培养的胎盘细胞（绒毛膜绒毛上皮细胞、朗汉斯细胞、霍夫鲍尔细胞及其子代细胞）进行了A和B血型抗原存在情况的研究，同时引入了直接和间接免疫荧光法。证实绒毛膜绒毛上皮细胞始终是无抗原的。朗汉斯细胞仅在其核膜内具有A和B移植抗原。但在霍夫鲍尔细胞中，可在细胞膜、核膜以及子代细胞的细胞质中发现抗原的存在。绒毛膜绒毛上皮层缺乏移植抗原也使得人类同种异体卵子能够着床并进一步发育。
