Matsuo O, Kato K, Matsuo C, Matsuo T
Anal Biochem. 1983 Nov;135(1):58-63. doi: 10.1016/0003-2697(83)90730-3.
The sensitive assay method of tissue plasminogen activator was established by an enzyme-immunoassay method, and discriminates tissue (nonurokinase) type plasminogen activator from urokinase. The sensitivity was 0.1 ng/assay tube, and the plasma concentration of tissue plasminogen activator in normal healthy subjects was 1.22 +/- 0.25 ng/ml. Distribution of tissue plasminogen activator was examined in normal tissue. A melanoma cell line was employed as cell culture medium for determination of tissue plasminogen activator.
采用酶免疫测定法建立了组织型纤溶酶原激活剂的灵敏检测方法，可区分组织（非尿激酶型）纤溶酶原激活剂和尿激酶。灵敏度为0.1 ng/检测管，正常健康受试者血浆中组织型纤溶酶原激活剂浓度为1.22±0.25 ng/ml。检测了正常组织中组织型纤溶酶原激活剂的分布。采用黑色素瘤细胞系作为细胞培养基来测定组织型纤溶酶原激活剂。
