Alpert S E, Pennington J E, Colten H R
Am Rev Respir Dis. 1984 Jan;129(1):66-71. doi: 10.1164/arrd.1984.129.1.66.
In order to assess the potential role of local production of complement in pulmonary host defenses against bacterial infection, this aspect of bronchoalveolar macrophage function was studied in guinea pigs challenged with Pseudomonas aeruginosa in an acute and chronic infection model. Acute infection resulted in an increase in bronchoalveolar macrophage cell number and an increase in synthesis and secretion rates for the second (C2) and fourth (C4) complement components per macrophage. Manipulation of the airway without introduction of Pseudomonas also increased synthesis of both C2 and C4 when studied 60 h after control solutions were administered. Pseudomonas aeruginosa delivered in agar beads to induce chronic inflammation resulted in specific stimulation of C2 and C4 synthesis at 2 wk and to a lesser extent at 4 wk postchallenge. This increase in local complement synthesis by bronchoalveolar macrophages, in addition to enhancing the local inflammatory response, may serve to facilitate recruitment of intravascular cellular and humoral mediators of host defense against bacterial infection.
为了评估补体的局部产生在肺部抵抗细菌感染的宿主防御中的潜在作用，在急性和慢性感染模型中，用铜绿假单胞菌攻击豚鼠，研究了支气管肺泡巨噬细胞功能的这一方面。急性感染导致支气管肺泡巨噬细胞数量增加，并且每个巨噬细胞的第二补体成分（C2）和第四补体成分（C4）的合成和分泌速率增加。在给予对照溶液60小时后进行研究时，不引入铜绿假单胞菌而对气道进行操作也增加了C2和C4的合成。以琼脂珠形式递送铜绿假单胞菌以诱导慢性炎症，在攻击后2周导致C2和C4合成的特异性刺激，在4周时刺激程度较小。支气管肺泡巨噬细胞局部补体合成的这种增加，除了增强局部炎症反应外，可能有助于促进针对细菌感染的宿主防御的血管内细胞和体液介质的募集。
