Brown C F, Osmond D H
Clin Sci (Lond). 1984 May;66(5):533-9. doi: 10.1042/cs0660533.
Cryoactivation of human plasma 'prorenin' was followed for 24 h at -4 degrees C. Chromogenic assays were used to determine factor XII (FXII), FXIIa, prekallikrein and kallikrein in relation to the observed cold-induced increase in plasma renin activity (PRA). Bradykinin activity was also determined using the rat uterus bioassay. PRA increased rapidly and became significantly higher after just 6 h of cryoactivation, by which time prekallikrein had almost disappeared, while kallikrein and kinin levels increased. In contrast, FXII did not change notably, but some FXIIa was indeed formed. The bacteriostat neomycin sulphate did not affect the course of cryoactivation, but did block the dextran sulphate- and kaolin-induced activation of prekallikrein and FXII respectively, and was therefore omitted. Thus cryoactivation of prorenin is accompanied by, and may depend upon, the activation of FXII and prekallikrein, supporting other evidence in favour of this hypothesis.
在-4℃下对人血浆“肾素原”进行24小时的低温激活。采用发色底物法测定与观察到的低温诱导血浆肾素活性（PRA）增加相关的因子XII（FXII）、FXIIa、前激肽释放酶和激肽释放酶。还使用大鼠子宫生物测定法测定缓激肽活性。低温激活后PRA迅速增加，仅6小时后就显著升高，此时前激肽释放酶几乎消失，而激肽释放酶和激肽水平增加。相比之下，FXII没有明显变化，但确实形成了一些FXIIa。抑菌剂硫酸新霉素不影响低温激活过程，但分别阻断了硫酸葡聚糖和高岭土诱导的前激肽释放酶和FXII的激活，因此被省略。因此，肾素原的低温激活伴随着FXII和前激肽释放酶的激活，并且可能依赖于它们的激活，这支持了支持该假说的其他证据。
