Miyake A, Tsaka K, Sakumoto T, Kawamura Y, Aono T, Kurachi K
Endocrinol Jpn. 1983 Aug;30(4):509-12. doi: 10.1507/endocrj1954.30.509.
The effects of norepinephrine (NE) on the release of luteinizing hormone (LH) and LH-releasing hormone (LH-RH) were examined in a sequential double chamber perifusion system by perfusing the medio-basal hypothalamus (MBH) and/or pituitaries excised from normal female rats in diestrus. When the pituitary alone or in sequence with the MBH was perifused with medium containing 200 micrograms/ml of NE, the concentration of LH in the efflux was significantly (p less than 0.05) increased to the level 50-150% over the pre-injection control, whereas medium without NE had no effect. Perifusion of the pituitary in sequence with the MBH produced a higher peak level of LH than that attained by the perifusion of the pituitary alone. Administration of NE induced a significant increase in LH-RH release from the MBH, although the medium alone had no effect. These data suggest that NE induces LH release from the pituitary both by increasing LH-RH release from the MBH and by its direct action on the pituitary.
通过在连续双室灌流系统中灌注从处于间情期的正常雌性大鼠切除的中基底下丘脑（MBH）和/或垂体，研究了去甲肾上腺素（NE）对促黄体生成素（LH）和促黄体生成素释放激素（LH-RH）释放的影响。当单独垂体或与MBH顺次灌注含200微克/毫升NE的培养基时，流出液中LH的浓度显著（p<0.05）升高至注射前对照水平的50-150%，而不含NE的培养基则无作用。与单独灌注垂体相比，垂体与MBH顺次灌注产生的LH峰值水平更高。给予NE可诱导MBH释放LH-RH显著增加，尽管单独培养基无作用。这些数据表明，NE通过增加MBH释放LH-RH及其对垂体的直接作用来诱导垂体释放LH。
