大肠杆菌K-12溶血磷脂酶L2编码基因的鉴定与克隆。

Identification and cloning of the gene coding for lysophospholipase L2 of E. coli K-12.

作者信息

Homma H, Kobayashi T, Ito Y, Kudo I, Inoue K, Ikeda H, Sekiguchi M, Nojima S

出版信息

J Biochem. 1983 Dec;94(6):2079-81. doi: 10.1093/oxfordjournals.jbchem.a134566.

DOI:10.1093/oxfordjournals.jbchem.a134566

Abstract

E. coli bearing hybrid plasmid pKOl (Oeda et al. (1981) Mol. Gen. Genet. 184, 191-199) expressed a large amount of lysophospholipase L2 activity. When a mutant which was defective in lysophospholipase L2 activity was transformed with plasmid pKOl, it overproduced lysophospholipase L2 activity. The gene responsible for the lysophospholipase L2 activity was designated as pld B. On the same hybrid plasmid another gene (pld A) coding for detergent-resistant phospholipase A (DR-phospholipase A) was also identified. These facts together with the results of a Pl transduction experiment revealed that the pld B gene must be between the pld A and met E genes on the E. coli chromosome.

摘要

携带杂交质粒pKOl的大肠杆菌（Oeda等人，（1981年），《分子与普通遗传学》，第184卷，第191 - 199页）表达出大量溶血磷脂酶L2活性。当用质粒pKOl转化缺乏溶血磷脂酶L2活性的突变体时，它过量产生溶血磷脂酶L2活性。负责溶血磷脂酶L2活性的基因被命名为pld B。在同一杂交质粒上还鉴定出另一个编码抗去污剂磷脂酶A（DR - 磷脂酶A）的基因（pld A）。这些事实以及P1转导实验的结果表明，pld B基因必定位于大肠杆菌染色体上的pld A基因和met E基因之间。