Pointis G, Rao B, Latreille M T, Cedard L
J Steroid Biochem. 1984 Jan;20(1):525-8. doi: 10.1016/0022-4731(84)90266-8.
Short-term primary culture of Leydig cells were prepared from 18 day old fetal mouse testes. The cells were cultured in a defined medium supplemented with 1% fetal calf serum, EGF and Insulin. The cells rapidly attached to the plastic culture dish. Seventy to eighty percent of the firmly attached cells stained positively for 3 beta-HSD activity and gradually assumed a flattened epitheloid appearance. The functional activity of these cells in terms of testosterone production and hCG-responsiveness was maintained for 2 days. There was a significant effect of plating density. Pre-culture (24 h) of fetal Leydig cells in the presence of 100 mIU hCG desensitized these cells to a subsequent stimulation by hCG. This is the first report of a short-term primary culture of fetal Leydig cells which demonstrates the maintenance of androgenic activity of these cells in vitro.
从18日龄胎鼠睾丸制备睾丸间质细胞的短期原代培养物。细胞在添加有1%胎牛血清、表皮生长因子（EGF）和胰岛素的限定培养基中培养。细胞迅速附着于塑料培养皿。70%至80%牢固附着的细胞3β-羟基类固醇脱氢酶（3β-HSD）活性染色呈阳性，并逐渐呈现扁平上皮样外观。这些细胞在睾酮产生和对人绒毛膜促性腺激素（hCG）反应性方面的功能活性维持了2天。接种密度有显著影响。在100 mIU hCG存在下对胎儿睾丸间质细胞进行预培养（24小时）会使这些细胞对随后的hCG刺激不敏感。这是关于胎儿睾丸间质细胞短期原代培养的首次报告，证明了这些细胞在体外雄激素活性的维持。
