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[利用荧光胺测定微生物悬浮液中的伯胺和蛋白质]

[Determination of primary amines and protein by using fluorescamine in microorganism suspensions].

作者信息

Poglazova M N, Pomoshchnikova N A, Meĭsel' M N

出版信息

Mikrobiologiia. 1984 Jan-Feb;53(1):28-32.

PMID:6369082
Abstract

The paper describes a fluorimetric technique for determining the overall amount of primary amines and protein using fluorescamine directly in suspensions of yeast and bacterial microorganisms. The optimal conditions are selected for the reaction. The technique is highly sensitive and has an advantage over biochemical methods when one has to make many assays of amine containing compounds in biological material. The technique can find wide application in industrial practice.

摘要

本文描述了一种荧光测定技术,可直接在酵母和细菌微生物悬浮液中使用荧光胺来测定伯胺和蛋白质的总量。选择了该反应的最佳条件。该技术灵敏度高,当需要对生物材料中的含胺化合物进行多次测定时,比生化方法更具优势。该技术可在工业实践中得到广泛应用。

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