Bergsten P, Hellman B
Endocrinology. 1984 May;114(5):1854-9. doi: 10.1210/endo-114-5-1854.
The problem of how glucose affects the intracellular (La3+-nondisplaceable) calcium content of pancreatic beta-cells was approached by combining measurements of 45Ca in ob/ob-mouse islets loaded to isotopic equilibrium with determinations of calcium using electrothermal atomic absorption spectroscopy. Whereas short term changes of the glucose concentration induced marked alterations of insulin release, the islet content of intracellular 45Ca was remarkably stable. The chronic actions of glucose differed from the acute ones in being readily demonstrable and sometimes resulting even in a suppression of the calcium content. Thus, after 7 days of culture in 20 mM glucose, the amount of intracellular calcium was actually lower than when the islets were cultured at 5.5 mM glucose. The long term effect of glucose in suppressing the islet content of intracellular calcium was associated with degranulation and loss of immunoreactive insulin, indicated both from staining of the beta-cells and measurements of the extracted hormone by RIA. The previously unknown ability of glucose to suppress the islet content of intracellular calcium may consequently result from mobilization of the secretory granules.
通过将对ob/ob小鼠胰岛中45Ca的测量（加载到同位素平衡状态）与使用电热原子吸收光谱法测定钙相结合，研究了葡萄糖如何影响胰腺β细胞内（镧系元素不可置换）钙含量的问题。虽然葡萄糖浓度的短期变化会引起胰岛素释放的显著改变，但细胞内45Ca的胰岛含量却非常稳定。葡萄糖的慢性作用与急性作用不同，其作用易于显现，有时甚至会导致钙含量的降低。因此，在20 mM葡萄糖中培养7天后，细胞内钙的含量实际上低于胰岛在5.5 mM葡萄糖中培养时的含量。葡萄糖抑制细胞内钙的胰岛含量的长期作用与脱颗粒和免疫反应性胰岛素的丢失有关，这从β细胞的染色和通过放射免疫分析法对提取的激素的测量中都可以看出。葡萄糖抑制细胞内钙的胰岛含量这一先前未知的能力可能是由于分泌颗粒的动员所致。
