• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

Separation and characterization of pyrimidine deoxyribonucleoside 2'-hydroxylase and thymine 7-hydroxylase from Aspergillus nidulans.

作者信息

Shaffer P M, Cairns J R, Dorman D C, Lott A M

出版信息

Int J Biochem. 1984;16(4):429-34. doi: 10.1016/0020-711x(84)90143-5.

DOI:10.1016/0020-711x(84)90143-5
PMID:6370754
Abstract

Partially purified preparations from Aspergillus nidulans were shown to catalyze two alpha-ketoglutarate dependent dioxygenase reactions: the pyrimidine deoxyribonucleoside 2'-hydroxylase (EC 1.14.11.3) and the thymine 7-hydroxylase (EC 1.14.11.6) reactions. These reactions showed an absolute requirement for alpha-ketoglutarate and molecular oxygen and were stimulated by Fe(II), ascorbate and catalase. Both reactions demonstrated a stoichiometry such that for each mole of substrate (deoxyribonucleoside or pyrimidine) hydroxylated one mole of CO2 was produced from alpha-ketoglutarate. These two activities were separated using DEAE-Sephacel chromatography.

摘要

相似文献

1
Separation and characterization of pyrimidine deoxyribonucleoside 2'-hydroxylase and thymine 7-hydroxylase from Aspergillus nidulans.
Int J Biochem. 1984;16(4):429-34. doi: 10.1016/0020-711x(84)90143-5.
2
Thymine 7-hydroxylase and pyrimidine deoxyribonucleoside 2' -hydroxylase activities in Rhodotorula glutinis.粘红酵母中的胸腺嘧啶7-羟化酶和嘧啶脱氧核糖核苷2'-羟化酶活性
J Biol Chem. 1978 Sep 25;253(18):6511-5.
3
Regulation of pyrimidine salvage in Aspergillus nidulans: a role for the major regulatory gene are A mediating nitrogen metabolite repression.构巢曲霉中嘧啶补救途径的调控：主要调控基因are A在介导氮代谢物阻遏中的作用。
Mol Gen Genet. 1984;198(2):139-45. doi: 10.1007/BF00328713.
4
Markedly different ascorbate dependencies of the sequential alpha-ketoglutarate dioxygenase reactions catalyzed by an essentially homogeneous thymine 7-hydroxylase from Rhodotorula glutinis.来自粘红酵母的基本纯一的胸腺嘧啶7-羟化酶催化的连续α-酮戊二酸双加氧酶反应具有明显不同的抗坏血酸盐依赖性。
J Biol Chem. 1983 Sep 10;258(17):10551-7.
5
Uracil's uncoupling of the decarboxylation of alpha-ketoglutarate in the thymine 7-hydroxylase reaction of Neurospora crassa.尿嘧啶在粗糙脉孢菌胸腺嘧啶7-羟化酶反应中对α-酮戊二酸脱羧作用的解偶联。
J Biol Chem. 1981 Jun 25;256(12):6098-101.
6
Stoichiometry of the pyrimidine deoxyribonucleoside 2'-hydroxylase reaction and of the conversions of 5-hydroxymethyluracil to 5-formyluracil and of the latter to uracil-5-carboxylic acid.嘧啶脱氧核糖核苷2'-羟化酶反应的化学计量关系以及5-羟甲基尿嘧啶向5-甲酰基尿嘧啶的转化和后者向尿嘧啶-5-羧酸的转化。
Biochemistry. 1972 May 23;11(11):2172-6. doi: 10.1021/bi00761a025.
7
Purification and characterization of the FeII- and alpha-ketoglutarate-dependent xanthine hydroxylase from Aspergillus nidulans.构巢曲霉中依赖FeII和α-酮戊二酸的黄嘌呤羟化酶的纯化与特性分析
Biochemistry. 2007 May 8;46(18):5293-304. doi: 10.1021/bi700065h. Epub 2007 Apr 13.
8
Novel phacB-encoded cytochrome P450 monooxygenase from Aspergillus nidulans with 3-hydroxyphenylacetate 6-hydroxylase and 3,4-dihydroxyphenylacetate 6-hydroxylase activities.来自构巢曲霉的新型phacB编码的细胞色素P450单加氧酶，具有3-羟基苯乙酸6-羟化酶和3,4-二羟基苯乙酸6-羟化酶活性。
Eukaryot Cell. 2007 Mar;6(3):514-20. doi: 10.1128/EC.00226-06. Epub 2006 Dec 22.
9
Catalysis of three sequential dioxygenase reactions by thymine 7-hydroxylase.胸腺嘧啶7-羟化酶对三个连续双加氧酶反应的催化作用。
Arch Biochem Biophys. 1973 Nov;159(1):180-7. doi: 10.1016/0003-9861(73)90443-8.
10
Structure function and engineering of multifunctional non-heme iron dependent oxygenases in fungal meroterpenoid biosynthesis.真菌生源二萜中多功能非血红素铁依赖型加氧酶的结构功能与工程化
Nat Commun. 2018 Jan 9;9(1):104. doi: 10.1038/s41467-017-02371-w.

引用本文的文献

1
Fe(II)/alpha-ketoglutarate hydroxylases involved in nucleobase, nucleoside, nucleotide, and chromatin metabolism.参与核碱基、核苷、核苷酸和染色质代谢的亚铁离子/α-酮戊二酸羟化酶。
Dalton Trans. 2008 Oct 14(38):5132-42. doi: 10.1039/b803512a. Epub 2008 Jun 27.
2
Regulation of pyrimidine salvage in Aspergillus nidulans: a role for the major regulatory gene are A mediating nitrogen metabolite repression.构巢曲霉中嘧啶补救途径的调控：主要调控基因are A在介导氮代谢物阻遏中的作用。
Mol Gen Genet. 1984;198(2):139-45. doi: 10.1007/BF00328713.