Azab M E, Safer E H, Ghaffar F M
Folia Parasitol (Praha). 1984;31(1):93-6.
A modified technique for the preparation of a cercarial microscopic slide antigen for use in IFAT, that could be stored at -20 degrees C for 6 months is described. Cercariae were deposited by cooling, fixed in 0.5% buffered formaline, mixed with 7% egg albumin, dropped on slides and dried. Before use, antigen was post-fixed in absolute methyl alcohol. Evans blue was used as a counterstain. The antigen was evaluated with known human positive and negative sera, also with sera from normal individuals and others residing in endemic areas and exposed to infections with schistosomiasis; as well as with sera of patients with other helminthic infections (strongyloidiasis, ankylostomiasis, ascariasis and microfilariasis). It indicated a sensitivity of 83% and a specificity of 61.5%.
描述了一种用于制备用于间接荧光抗体试验（IFAT）的尾蚴显微玻片抗原的改良技术，该抗原可在-20℃下储存6个月。通过冷却沉积尾蚴，用0.5%缓冲福尔马林固定，与7%卵白蛋白混合，滴在玻片上并干燥。使用前，抗原用无水甲醇后固定。伊文思蓝用作复染剂。用已知的人类阳性和阴性血清、正常个体以及居住在流行区并接触血吸虫感染的其他人的血清，以及患有其他蠕虫感染（类圆线虫病、钩虫病、蛔虫病和丝虫病）患者的血清对该抗原进行评估。其显示敏感性为83%，特异性为61.5%。
