Cerra R F, Haywood-Reid P L, Barondes S H
J Cell Biol. 1984 Apr;98(4):1580-9. doi: 10.1083/jcb.98.4.1580.
An affinity-purified antibody preparation raised against a beta-galactoside-binding lectin from bovine lung was used to localize a similar lectin in rat lung by immunofluorescence and by electron microscopy after on-grid staining visualized with colloidal gold conjugated second antibody. The endogenous mammalian lectin was found in smooth muscle cells and squamous alveolar epithelial (type I) cells and was concentrated extracellularly in elastic fibers of pulmonary parenchyma and blood vessels. The extracellular localization of this lectin suggests that it, like others, functions by interaction with extracellular glycoconjugates.
一种针对牛肺中β-半乳糖苷结合凝集素制备的亲和纯化抗体制剂,通过免疫荧光以及在使用胶体金偶联二抗进行网格染色后通过电子显微镜,用于在大鼠肺中定位类似的凝集素。发现内源性哺乳动物凝集素存在于平滑肌细胞和鳞状肺泡上皮(I型)细胞中,并在肺实质和血管的弹性纤维中细胞外聚集。这种凝集素的细胞外定位表明,它与其他凝集素一样,通过与细胞外糖缀合物相互作用发挥功能。