内源性哺乳动物凝集素定位于肺弹性纤维的细胞外。
Endogenous mammalian lectin localized extracellularly in lung elastic fibers.
作者信息
Cerra R F, Haywood-Reid P L, Barondes S H
出版信息
J Cell Biol. 1984 Apr;98(4):1580-9. doi: 10.1083/jcb.98.4.1580.
Abstract
An affinity-purified antibody preparation raised against a beta-galactoside-binding lectin from bovine lung was used to localize a similar lectin in rat lung by immunofluorescence and by electron microscopy after on-grid staining visualized with colloidal gold conjugated second antibody. The endogenous mammalian lectin was found in smooth muscle cells and squamous alveolar epithelial (type I) cells and was concentrated extracellularly in elastic fibers of pulmonary parenchyma and blood vessels. The extracellular localization of this lectin suggests that it, like others, functions by interaction with extracellular glycoconjugates.
摘要
一种针对牛肺中β-半乳糖苷结合凝集素制备的亲和纯化抗体制剂，通过免疫荧光以及在使用胶体金偶联二抗进行网格染色后通过电子显微镜，用于在大鼠肺中定位类似的凝集素。发现内源性哺乳动物凝集素存在于平滑肌细胞和鳞状肺泡上皮（I型）细胞中，并在肺实质和血管的弹性纤维中细胞外聚集。这种凝集素的细胞外定位表明，它与其他凝集素一样，通过与细胞外糖缀合物相互作用发挥功能。
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本文引用的文献
1
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J Biol Chem. 1981 Jan 10;256(1):390-4.
2
Postnatal development of rat lung. Changes in lung lectin, elastin, acetylcholinesterase and other enzymes.大鼠肺的出生后发育。肺凝集素、弹性蛋白、乙酰胆碱酯酶及其他酶的变化。
Biochem J. 1980 Apr 15;188(1):1-8. doi: 10.1042/bj1880001.
3
Purification and properties of lung lectin. Rat lung and human lung beta-galactoside-binding proteins.肺凝集素的纯化与性质。大鼠肺和人肺β-半乳糖苷结合蛋白。
Biochem J. 1980 Apr 1;187(1):123-9. doi: 10.1042/bj1870123.
4
Externalization of an endogenous chicken muscle lectin with in vivo development.内源性鸡肌肉凝集素随体内发育的外化。
J Cell Biol. 1981 Nov;91(2 Pt 1):568-72. doi: 10.1083/jcb.91.2.568.
5
6
Quantitation of two endogenous lactose-inhibitable lectins in embryonic and adult chicken tissues.胚胎和成年鸡组织中两种内源性乳糖抑制凝集素的定量分析。
J Cell Biol. 1982 Jan;92(1):23-7. doi: 10.1083/jcb.92.1.23.
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Secretion of endogenous lectin by chicken intestinal goblet cells.鸡肠道杯状细胞内源性凝集素的分泌
J Cell Biol. 1982 Jan;92(1):28-33. doi: 10.1083/jcb.92.1.28.
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