Benbouzid A, Guinet R, Cloppet H
J Chromatogr. 1984 Mar 9;306:117-24. doi: 10.1016/s0378-4347(00)80875-0.
Serological methods can be applied to the diagnosis of systemic candidiasis, but cell wall mannans can detect antibodies occurring in several cases unrelated to candidiasis. The present study proposes a procedure for the preparation of specific antigens obtained from an experimental infectious model. The specific immunoglobulins were obtained from rabbits with chronic systemic candidiasis. After precipitation by ammonium sulfate and purification by ion-exchange chromatography on DEAE-Sephadex A-50, these immunoglobulins were fixed on cyanogen bromide-activated Sepharose 4B and used for the preparation of an affinity chromatography column. This column allowed isolation of specifically bound fractions of Candida albicans soluble antigens. When analyzed by quantitative immunoelectrophoresis with a polyvalent hyperimmune antiserum, these fractions showed eight precipitation lines, whereas the complete soluble antigen exhibited 48 lines. Possible applications of these antigens to specific serodiagnosis of systemic candidiasis are being evaluated.
血清学方法可用于系统性念珠菌病的诊断，但细胞壁甘露聚糖能检测到在一些与念珠菌病无关的病例中出现的抗体。本研究提出了一种从实验性感染模型中制备特异性抗原的方法。特异性免疫球蛋白取自患有慢性系统性念珠菌病的兔子。经硫酸铵沉淀并在DEAE-葡聚糖A-50上进行离子交换色谱纯化后，将这些免疫球蛋白固定在溴化氰活化的琼脂糖4B上，用于制备亲和色谱柱。该柱可分离白色念珠菌可溶性抗原的特异性结合组分。当用多价超免疫抗血清进行定量免疫电泳分析时，这些组分显示出8条沉淀线，而完整的可溶性抗原则显示出48条沉淀线。目前正在评估这些抗原在系统性念珠菌病特异性血清诊断中的可能应用。
