采用酶免疫测定法（EIA）的四层改良法检测临床标本中的人型支原体抗原。

Detection of Mycoplasma hominis antigen in clinical specimens by using a four-layer modification of enzyme immunoassay (EIA).

作者信息

Miettinen A, Turunen H, Paavonen J, Jansson E, Leinikki P

出版信息

J Immunol Methods. 1984 Apr 27;69(2):267-75. doi: 10.1016/0022-1759(84)90324-7.

Abstract

A 4-layer modification of enzyme immunoassay (EIA) was developed for the detection of Mycoplasma hominis antigen in clinical specimens. Microtiter plates were sensitized with rabbit anti-mycoplasma immunoglobulin, guinea pig anti-mycoplasma immunoglobulin was used as the secondary antibody, and horseradish peroxidase-conjugated anti-guinea pig immunoglobulin was used as the indicator antibody. The specificity of the assay was confirmed by using guinea pig immunoglobulins from preimmunization sera. The sensitivity of the assay is down to 10 ng/ml of antigen protein. Marked cross-reactivity was demonstrated for different strains within the species M. hominis, whereas the other genital mycoplasma species tested showed no reactivity in the assay. A comparison was made of EIA and conventional culture of vaginal specimens from 24 women. All 6 specimens positive by culture were also positive for M. hominis antigen by EIA. Antigen detection by EIA is a sensitive, rapid and simple method for the detection of M. hominis in clinical specimens.

摘要

开发了一种用于检测临床标本中人型支原体抗原的四层酶免疫测定（EIA）方法。用兔抗支原体免疫球蛋白致敏微量滴定板，用豚鼠抗支原体免疫球蛋白作为二抗，用辣根过氧化物酶标记的抗豚鼠免疫球蛋白作为指示抗体。通过使用来自免疫前血清的豚鼠免疫球蛋白来确认该测定的特异性。该测定的灵敏度低至10 ng/ml抗原蛋白。人型支原体种内不同菌株表现出明显的交叉反应性，而测试的其他生殖支原体种在该测定中无反应性。对24名女性阴道标本的EIA和传统培养进行了比较。培养阳性的所有6个标本通过EIA检测人型支原体抗原也呈阳性。EIA抗原检测是一种检测临床标本中人型支原体的灵敏、快速且简单的方法。