人型支原体抗原
Antigens of Mycoplasma hominis.
作者信息
Brown M B, Minion F C, Davis J K, Pritchard D G, Cassell G H
出版信息
Sex Transm Dis. 1983 Oct-Dec;10(4 Suppl):247-55.
The antigens of Mycoplasma hominis have previously been defined by means of functional assays with rabbit antisera and have been found to be predominantly protein. In this study, sera from humans as well as those from rabbits were shown to recognize protein antigens of M. hominis in an enzyme-linked immunosorbent assay (ELISA) that depended on antigen-binding capacity rather than biologic function. Although the ELISA was more independent of the strain of M. hominis used as antigen than was the mycoplasmacidal test, the use of no single strain allowed detection of more than 87% of positive sera; this observation suggests strain heterogeneity. In contrast to the results of other studies, common surface antigens among the strains of M. hominis used were demonstrated by immunoblot analysis of intact colonies.
人型支原体的抗原先前已通过兔抗血清功能测定法得以界定，且发现主要为蛋白质。在本研究中，在一种依赖抗原结合能力而非生物学功能的酶联免疫吸附测定（ELISA）中，人血清以及兔血清均显示可识别出人型支原体的蛋白质抗原。尽管ELISA相比支原体杀菌试验，对抗原所用人型支原体菌株的依赖性更低，但使用单一菌株时，阳性血清的检出率均不超过87%；这一观察结果提示存在菌株异质性。与其他研究结果相反，通过对完整菌落进行免疫印迹分析，证实了所用人型支原体菌株之间存在共同的表面抗原。
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