Bitko S A, Savel'ev E P, Petrov G I
Prikl Biokhim Mikrobiol. 1984 Mar-Apr;20(2):285-9.
A method of determining aminosaccharides (muramic acid, glucosamine and galactosamine) by means of a carbohydrate analyzer "Biotronic" using the cation-exchange resin DC-6 A ("Durrum") was developed. Chromatographic conditions correspond to the conditions of neutral sugar analysis on a column with DCh-4 resin, that enables after a slight modification of the analyzer to pass from the determining of aminosaccharides to the determining of neutral sugars. The method was used for determining the carbohydrate composition of streptococcus cell walls. The results obtained allow to conclude that using this method one can get more information on the hydrocarbon composition of various biological objects than using the method of aminosaccharide determining by means of aminoacid anylyzer, which is widely in practice nowadays.
开发了一种使用阳离子交换树脂DC - 6A（“Durrum”）通过碳水化合物分析仪“Biotronic”测定氨基糖（胞壁酸、葡萄糖胺和半乳糖胺）的方法。色谱条件与在装有DCh - 4树脂的柱上进行中性糖分析的条件一致，这使得在对分析仪进行轻微修改后，能够从氨基糖的测定转换为中性糖的测定。该方法用于测定链球菌细胞壁的碳水化合物组成。所得结果表明，与目前在实践中广泛使用的通过氨基酸分析仪测定氨基糖的方法相比，使用该方法可以获得更多关于各种生物物体碳水化合物组成的信息。
